The accumulation and effect of rare earth element neodymium on the root of rice seedlings

Neodymium (Nd) potentially threatens ecological equilibrium for its wide usage in industries. In this study, the accumulation and effect of Nd on roots were investigated in the rice seedlings (Oryza sativa L.) exposed to different concentrations of Nd (0, 1, 10, 100, and 1000 μM). The toxic effect of Nd on rice growth was observed at the higher concentration, but the positive effects were found at the lower concentration. The accumulation of Nd was present in six different chemical forms, and the insoluble phosphate and oxalate Nd were the major forms of Nd in the roots. In addition, Nd was accumulated in the soluble fractions, organelles, and cell walls of rice seedlings, and the root cell wall was a major Nd sink site. The result of Fourier transform infrared spectrometer spectral analysis indicated that the functional groups of -OH and C-OH were the major binding sites of Nd in the cell wall of roots. Moreover, the level of reactive oxygen species (ROS) and the activity of catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) were significantly increased with the increase of Nd concentration. The enhanced antioxidant capacity also played an important role in Nd detoxification of rice seedlings. In all, the results indicated that forming of inactive oxalate or phosphate and efficient sequestration into the root cell wall was a key process in Nd accumulation and detoxification of rice seedlings.