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SLCO4A1-AS1 regulates laryngeal squamous cell carcinoma cell phenotypes via the Wnt pathway

Feng Zhang1, Dehong Mao1, Zhongmei He1

  • 1Department of Otolaryngology, Yongchuan Hospital (T.C.M) of Chongqing Medical University, Chongqing, China.

Oral Diseases
|April 29, 2021
PubMed
Abstract

Insights

Solute carrier organic anion transporter family member 4A1 antisense RNA 1 (SLCO4A1-AS1) promotes laryngeal squamous cell carcinoma (LSCC) growth. It upregulates SETD7, activating the Wnt/β-catenin pathway and inhibiting apoptosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Laryngeal squamous cell carcinoma (LSCC) is a common head and neck cancer.
  • Long non-coding RNAs (lncRNAs) are implicated in LSCC pathogenesis.
  • The role of SLCO4A1-AS1 in LSCC requires further investigation.

Purpose of the Study:

  • To elucidate the function of SLCO4A1-AS1 in LSCC.
  • To investigate the molecular mechanisms underlying SLCO4A1-AS1's role in LSCC.

Main Methods:

  • Cell viability and proliferation assays (CCK-8, colony formation).
  • Apoptosis assays (flow cytometry, TUNEL).
  • Subcellular fractionation, luciferase reporter assays to confirm molecular interactions.

Main Results:

  • SLCO4A1-AS1 is overexpressed in LSCC tissues and cells.
  • Silencing SLCO4A1-AS1 inhibits LSCC cell proliferation and promotes apoptosis.
  • SLCO4A1-AS1 acts as a ceRNA for miR-7855-5p, upregulating SETD7 and activating the Wnt/β-catenin pathway.

Conclusions:

  • SLCO4A1-AS1 promotes LSCC proliferation and inhibits apoptosis.
  • This occurs via SETD7 upregulation in a miR-7855-5p dependent manner.
  • Activation of the Wnt/β-catenin pathway is a key downstream effect.
Keywords:
LSCCSETD7SLCO4A1-AS1miR-7855-5p

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