Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

638
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
638
Labeling DNA Probes03:31

Labeling DNA Probes

8.7K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The subpleural pulmonary microvasculature in newborn yak (Bos grunniens).

Veterinary research communications·2008
Same author

Experimental confirmation of potential swept source optical coherence tomography performance limitations.

Applied optics·2008
Same author

A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice.

Plant physiology·2008
Same author

[Spatial and temporal changes of palatal cell proliferation and cell apoptosis of retinoic acid induced mouse cleft palate in different embryonic stages].

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology·2008
Same author

Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties.

Developmental and comparative immunology·2008
Same author

Non-Gaussian statistics and superdiffusion in a driven-dissipative dusty plasma.

Physical review. E, Statistical, nonlinear, and soft matter physics·2008

Related Experiment Video

Updated: Nov 7, 2025

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
10:38

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

14.1K

A simple strategy for constructing PET fluorescent probe and its application in hypochlorite detection.

Xiaowen Zhang1, Fei Zhang1, Binsheng Yang1

  • 1Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|April 29, 2021
PubMed
Summary

Developing rapid and sensitive fluorescent probes is crucial for detecting hypochlorite (ClO-) in biological systems. This study presents a novel strategy using photoinduced electron transfer (PET) to create effective ClO- sensors.

Keywords:
Fluorescent probeHypochloritePhotoinduced electron transfer (PET)Rapid responseSimple strategy

More Related Videos

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
14:11

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Published on: June 10, 2021

6.4K
Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.5K

Related Experiment Videos

Last Updated: Nov 7, 2025

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
10:38

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

14.1K
Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
14:11

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Published on: June 10, 2021

6.4K
Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.5K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Chemical Biology

Background:

  • Hypochlorite (ClO-) plays a vital role in the immune system.
  • Developing rapid and efficient detection methods for ClO- is essential for biological and medical research.
  • Existing detection methods may lack sensitivity, speed, or specificity.

Purpose of the Study:

  • To propose a new strategy for constructing fluorescent probes for hypochlorite detection based on the photoinduced electron transfer (PET) mechanism.
  • To develop and evaluate novel fluorescent probes for sensitive and rapid detection of hypochlorite.
  • To demonstrate the applicability of the developed probe in biological samples.

Main Methods:

  • Design and synthesis of four fluorescent probes (TPA-NO2, TPA-2NO2, TPB-NO2, TPB-2NO2) based on the PET mechanism.
  • Investigation of the fluorescent response of the probes to hypochlorite.
  • Determination of the detection limit and response time of the most effective probe.
  • In vitro and in vivo imaging studies using the optimized probe in living cells and zebrafish.

Main Results:

  • Four novel fluorescent probes were successfully synthesized and characterized.
  • Probe TPB-NO2 exhibited significant fluorescence changes upon reaction with hypochlorite.
  • TPB-NO2 demonstrated a rapid response time (<90 s) and a low detection limit (0.36 μM).
  • The probe TPB-NO2 was successfully applied for hypochlorite detection in living cells and zebrafish, confirming its biological applicability.

Conclusions:

  • The proposed PET-based strategy is feasible for developing efficient fluorescent probes for hypochlorite detection.
  • The developed probe TPB-NO2 offers a promising tool for rapid and sensitive detection of hypochlorite in biological environments.
  • This work provides valuable guidance for the future design of advanced fluorescent sensors for reactive species.