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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Updated: Nov 7, 2025

Autofluorescence Imaging to Evaluate Cellular Metabolism
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Recent progress in developing fluorescent probes for imaging cell metabolites.

Shanni Hong1,2,3, Gregory T Pawel2, Renjun Pei3

  • 1Department of Medical Imaging Technology, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou, People's Republic of China.

Biomedical Materials (Bristol, England)
|April 29, 2021
PubMed
Summary
This summary is machine-generated.

Fluorescent probes now enable real-time tracking of key cellular metabolites like adenosine triphosphate (ATP) and nicotinamide adenine dinucleotide (NADH) in living cells. This review covers advancements in fluorescent probe technology for metabolite imaging.

Keywords:
fluorescent probesimagingmetabolites

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Analytical Chemistry

Background:

  • Cellular metabolites are vital for cellular functions but challenging to monitor in real-time within living cells.
  • Advances in fluorescent probe technology have significantly improved the ability to track these essential molecules.
  • Real-time imaging of metabolites is crucial for understanding cellular dynamics.

Purpose of the Study:

  • To review recent advancements in fluorescent probes for monitoring key cellular metabolites.
  • To highlight the application of these probes in both whole-cell and subcellular imaging.
  • To provide an overview of fluorescent probe development for metabolite tracking.

Main Methods:

  • Literature review of recent studies on fluorescent probes for metabolite detection.
  • Focus on probes designed for specific metabolites including ATP, cAMP, cGMP, NADH, ROS, sugars, CO, and NO.
  • Analysis of imaging techniques for whole-cell and subcellular metabolite visualization.

Main Results:

  • Development of sophisticated fluorescent probes allows for sensitive and specific detection of various metabolites.
  • Successful real-time imaging of metabolites like NADH and ATP has been achieved using advanced probes.
  • Probes enable visualization of metabolic dynamics at subcellular levels.

Conclusions:

  • Fluorescent probes are powerful tools for real-time monitoring of cellular metabolites.
  • Recent progress has expanded the range of trackable metabolites and improved imaging capabilities.
  • These advancements are critical for understanding cellular processes and disease mechanisms.