Embedded Immunodetection System for Fecal Occult Blood.

Area of Science:

• Biomedical Engineering
• Clinical Diagnostics
• Analytical Chemistry

Background:

• Fecal occult blood (FOB) testing is crucial for early detection of gastrointestinal diseases.
• Existing methods may lack sensitivity, linearity, or stability for accurate quantitative analysis.
• A need exists for a rapid, reliable, and quantitative FOB detection system.

Purpose of the Study:

• To develop and validate a novel rapid test system for quantitative fecal occult blood detection.
• To assess the system's sensitivity, linearity, stability, and accuracy.
• To integrate hardware and Python-based software for automated analysis.

Main Methods:

• Development of a rapid test system utilizing immunochromatographic strips and immune response coloration.
• Implementation of Python-based software for main program control, polarization calibration, QR Code decoding, Bluetooth transmission, and image processing.
• Quantitative analysis of fecal occult blood concentration based on coloration intensity.

Main Results:

• The system demonstrated high sensitivity, linearity, and stability for FOB detection.
• Quantitative analysis achieved high accuracy with coefficients of variation (CV) below 3.7% for concentrations up to 400 ng/mL.
• Even at 1000 ng/mL, the system maintained a low CV of 7.41%.

Conclusions:

• The presented rapid test system provides a sensitive, linear, and stable platform for quantitative FOB detection.
• The integrated hardware and software approach ensures accurate and reliable diagnostic results.
• This system holds potential for improved early screening of gastrointestinal conditions.