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Updated: Nov 7, 2025
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Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Published on: June 3, 2019
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Differentiation of Capripox Viruses by Nanopore Sequencing
Kamal H Eltom1, Anna Christina Althoff2, Sören Hansen2
1Unit of Animal Health and Safety of Animal Products, Institute for Studies and Promotion of Animal Exports, University of Khartoum, Shambat 13314, Khartoum North, Sudan.
Vaccines
|April 30, 2021
Summary
Developing a rapid, field-applicable method for differentiating capripoxviruses (CaPV) is crucial for disease control. This study introduces a Nanopore sequencing approach for quick and accurate CaPV species identification, aiding outbreak management.
Area of Science:
- Virology
- Molecular Biology
- Veterinary Science
Background:
- Capripoxvirus (CaPV) causes significant economic losses in livestock.
- CaPV species (GPV, SPV, LSDV) are genetically similar, complicating diagnosis.
- Existing diagnostic methods lack the speed and specificity needed for field outbreaks.
Purpose of the Study:
- To develop a field-applicable method for differentiating CaPV species.
- To enable rapid and reliable diagnosis during disease outbreaks.
Main Methods:
- Whole genome sequencing using Nanopore technology.
- Creation of a local database for offline Basic Local Alignment Search Tool (BLAST).
- Analysis of specific genes (RPO30, P32, GPCR) for differentiation.
Main Results:
- Achieved high specificity: 98.04% for whole genome and 97.86% for RPO30 gene.
- Reduced total run time to approximately 2 hours.
- Demonstrated functionality with samples containing high host background DNA.
Conclusions:
- The developed Nanopore sequencing workflow provides a rapid and reliable method for CaPV differentiation.
- This approach is suitable for point-of-need application in resource-limited settings using mobile suitcase laboratories.
- Findings offer reliable differentiation options based on hardware, sample quality, and case capacity.
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