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Updated: Nov 7, 2025

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

Characterization of Liposomes Using Quantitative Phase Microscopy (QPM).

Jennifer Cauzzo¹, Nikhil Jayakumar², Balpreet Singh Ahluwalia²

¹Drug Transport and Delivery Research Group, Department of Pharmacy, Faculty of Health Sciences, University of Tromsø The Arctic University of Norway, N-9037 Tromsø, Norway.

|April 30, 2021

Summary

This summary is machine-generated.

Quantitative phase microscopy (QPM) offers a label-free method to characterize nanomedicine, overcoming limitations of traditional techniques. This advanced imaging accurately sizes and tracks liposomes, providing detailed insights into nanosystem behavior.

Keywords:

characterization label-free liposomes nanomedicine quantitative phase microscopy

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Area of Science:

Nanomedicine
Biotechnology
Materials Science

Background:

Characterizing nanomedicine and drug delivery systems requires advanced techniques.
Conventional methods like dynamic light scattering (DLS) and fluorescent assays have limitations including instability and loss of tracking.
Batch-mode analyses and fluorescent labeling pose challenges in nanosystem characterization.

Purpose of the Study:

To introduce quantitative phase microscopy (QPM) as a label-free, complementary characterization technique for nanosystems.
To address the limitations of traditional batch-mode analyses and fluorescent labeling.
To demonstrate QPM's capability in characterizing liposomes as model nanocarriers.

Main Methods:

Liposomes were immobilized in a non-dried setup for static imaging.
Off-axis phase microscopy was employed for imaging.
A phase-shifting algorithm was used for image reconstruction, achieving high spatial resolution.
Quantitative Phase Microscopy (QPM) was utilized as the primary characterization tool.

Main Results:

QPM successfully localized subdiffraction-limited liposomes.
The technique accurately estimated liposome size and tracked their integrity over time.
QPM enabled single-particle-based size distribution estimation, offering an alternative to batch analysis.
Label-free imaging provided high spatial resolution and stability.

Conclusions:

QPM overcomes drawbacks of conventional characterization methods for nanosystems.
The technique is a valuable complementary tool for analyzing nanomedicine properties and behavior.
QPM demonstrates potential for accurate sizing, tracking, and integrity assessment of nanocarriers like liposomes.