SPAD Leaf Greenness Index: Green Mass Yield Indicator of Maize (Zea mays L.), Genetic and Agriculture Practice Relationship
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Updated: Nov 7, 2025

Imaging and Analysis for Quantifying Maize (Zea mays) Abiotic Stress Phenotypes
Published on: March 28, 2025
Piotr Szulc1, Jan Bocianowski2, Kamila Nowosad3
1Department of Agronomy, Poznań University of Life Sciences, Dojazd 11, 60-632 Poznań, Poland.
Abstract:
The study presents the results of two field studies (Experiment I, Experiment II), whose aim was to assess the impact of agriculture factors on maize green mass and leaf greenness index (Soil and Plant Analysis Development, SPAD) in critical growth stages, as well as to determine the relationship between the SPAD index and the yield of green maize for ensiling. It was shown that thermal and humidity conditions in maize growing seasons determined the value of the SPAD leaf greenness index and the yield of maize harvested for silage. Row application of mineral fertilizer (N, NP) and selection of "stay-green" varieties guarantee a higher yield of maize green mass. Growing maize in direct sowing reduces chlorophyll content expressed in SPAD units, thereby reducing plant nitrogen condition, which significantly decreases the yield of biomass intended for silage. The SPAD leaf greenness index determined in critical stages of maize growth can be considered as a yield predictor of green mass for ensiling. The examined maize cultivars were divided into two groups on the basis of hierarchically grouping using the unweighted pair group method of arithmetic means. The first group comprised cultivars SY Cooky and Drim "stay-green," while the second one included cultivars ES Paroli "stay-green" and ES Palazzo.
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