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Updated: Nov 7, 2025

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
Published on: February 12, 2019
Recent 1D and 2D TD-NMR Pulse Sequences for Plant Science
Tatiana Monaretto1,2, Tiago Bueno Moraes3, Luiz Alberto Colnago2
1Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador São-Carlense, 400, São Carlos 13566-590, SP, Brazil.
Abstract:
Time domain nuclear magnetic resonance (TD-NMR) has been widely applied in plant science in the last four decades. Several TD-NMR instruments and methods have been developed for laboratory, green-house, and field studies. This mini-review focuses on the recent TD-NMR pulse sequences applied in plant science. One of the sequences measures the transverse relaxation time (T2) with minimal sample heating, using a lower refocusing flip angle and consequently lower specific absorption rate than that of conventional CPMG. Other sequences are based on a continuous wave free precession (CWFP) regime used to enhance the signal-to-noise ratio, to measure longitudinal (T1) and transverse relaxation time in a single shot experiment, and as alternative 2D pulse sequences to obtain T1-T2 and diffusion-T1 correlation maps. This review also presents some applications of these sequences in plant science.
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