LncMyoD Promotes Skeletal Myogenesis and Regulates Skeletal Muscle Fiber-Type Composition by Sponging miR-370-3p

Area of Science:

• Molecular Biology
• Muscle Physiology

Background:

• Skeletal muscle development is complex, involving precise regulation.
• Noncoding RNAs, including long noncoding RNAs (lncRNAs) and microRNAs (miRNAs), are increasingly recognized for their roles in myogenesis.

Purpose of the Study:

• To investigate the role of lncMyoD in myogenesis and myofiber-type composition.
• To elucidate the regulatory relationship between lncMyoD, miR-370-3p, and muscle fiber characteristics.

Main Methods:

• Utilized the C2C12 cell line as a model for muscle development studies.
• Investigated the regulatory interactions between lncMyoD and miR-370-3p.
• Analyzed the impact of miR-370-3p on myoblast proliferation, differentiation, and fiber-type transition.
• Identified acyl-Coenzyme A dehydrogenase, short/branched chain (ACADSB) as a target gene of miR-370-3p.

Main Results:

• lncMyoD was found to regulate myogenesis and alter myofiber-type composition.
• miR-370-3p, targeted by lncMyoD, promoted myoblast proliferation but inhibited differentiation.
• Inhibition of miR-370-3p promoted fast-twitch fiber transition.
• ACADSB was confirmed as a target of miR-370-3p, involved in differentiation and fiber-type transition.
• lncMyoD sponged miR-370-3p, and lncMyoD promoted fast-twitch fiber transition.

Conclusions:

• miR-370-3p plays a critical role in regulating myoblast differentiation and muscle fiber type transition.
• lncMyoD acts as a molecular sponge for miR-370-3p, influencing these processes.
• The lncMyoD/miR-370-3p/ACADSB axis is a key regulatory pathway in skeletal muscle development and fiber specification.