Dispersive Liquid-Liquid Microextraction (DLLME) and LC-MS/MS Analysis for Multi-Mycotoxin in Rice Bran: Method...

Area of Science:

• Food Chemistry
• Analytical Chemistry
• Food Safety

Background:

• Rice bran is a nutritious by-product used in functional foods.
• Rice bran is susceptible to contamination by various harmful mycotoxins.
• Accurate detection of multiple mycotoxins is crucial for food safety.

Purpose of the Study:

• To develop and validate a method for simultaneous detection of 12 mycotoxins in rice bran.
• To optimize the extraction and detection process for improved accuracy and efficiency.
• To ensure the safety of rice bran as a functional food ingredient.

Main Methods:

• Dispersive liquid-liquid microextraction (DLLME) for sample preparation.
• Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for sensitive detection.
• Response surface methodology (RSM) with Box-Behnken Design (BBD) for optimization.

Main Results:

• High extraction recoveries (63-120%) achieved with optimized DLLME.
• Excellent linearity (r² ≥ 0.990) and low limits of detection (0.5-50 ng g⁻¹).
• Method validated with recoveries from 70.2% to 99.4% and RSD below 1.28%.

Conclusions:

• The developed LC-MS/MS method is reliable for multi-mycotoxin analysis in rice bran.
• This method contributes to ensuring the safety and quality of rice bran-based products.
• Successful application to analyze 24 real rice bran samples demonstrates practical utility.