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Communication compartments in the gastrulating mouse embryo.

G H Kalimi¹, C W Lo

¹Biology Department, University of Pennsylvania, Philadelphia 19104.

The Journal of Cell Biology

|July 1, 1988

Summary

Gap junctional communication in mouse embryos reveals distinct compartments within germ layers during gastrulation. While germ layers are largely separate, ionic coupling persists between them, suggesting a role in embryonic development.

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Area of Science:

Developmental Biology
Cellular Communication
Embryogenesis

Background:

Gap junctional communication is crucial for embryonic development.
Understanding communication patterns during gastrulation is key to deciphering developmental processes.

Purpose of the Study:

To characterize gap junctional communication patterns in the 7.5-day mouse embryo during gastrulation.
To identify and define communication compartments within and between embryonic germ layers.

Main Methods:

Injected fluorescent tracers (Lucifer Yellow, carboxyfluorescein) to assess dye coupling.
Utilized thick section histology for detailed analysis of dye spread.
Monitored ionic coupling to evaluate communication across germ layers.

Main Results:

Cells within all three germ layers showed significant dye coupling, indicating widespread communication.
Dye spread was restricted between germ layers, defining them as separate communication compartments.
Partially restrictive, "box-like" communication domains were observed within the ectoderm and mesoderm.
Ionic coupling was detected between germ layers, despite apparent dye uncoupling.

Conclusions:

Partially restrictive communication compartments are associated with germ layer delamination in gastrulating mouse embryos.
Gap junctional communication is maintained across germ layer boundaries, suggesting a role in embryonic patterning and development.

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