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A Compact Detection Platform Based on Gradient Guided-Mode Resonance for Colorimetric and Fluorescence Liquid Assay
Jing-Jhong Gao1, Ching-Wei Chiu1, Kuo-Hsing Wen2
1Department of Mechanical Engineering, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan.
Sensors (Basel, Switzerland)
|April 30, 2021
Summary
A compact spectral detection system using a gradient grating period guided-mode resonance (GGP-GMR) filter enables sensitive quantification of fluorescent and colorimetric assays. This technology shows promise for point-of-care applications with its small size and high performance.
Area of Science:
- Optical Engineering
- Biomedical Sensing
- Analytical Chemistry
Background:
- Traditional spectral detection systems can be bulky and expensive.
- There is a need for compact, sensitive detection systems for point-of-care diagnostics.
Purpose of the Study:
- To develop and demonstrate a compact spectral detection system for fluorescent and colorimetric assays.
- To evaluate the system's performance against a commercial spectrometer.
Main Methods:
- Integration of a gradient grating period guided-mode resonance (GGP-GMR) filter with a charge-coupled device.
- Direct measurement of fluorescence emission and spectral analysis.
- Quantification of albumin (fluorescent and colorimetric) and creatinine (colorimetric) assays.
Main Results:
- The GGP-GMR filter achieved a spectral detection range of 500-700 nm in a <2.5 mm device.
- The system accurately detected peak wavelength and intensity for fluorescent assays.
- Achieved limits of detection (LODs) of 40.99 mg/L (fluorescent albumin), 398 mg/L (colorimetric albumin), and 25.49 mg/L (colorimetric creatinine).
Conclusions:
- The proposed compact spectral detection system demonstrates high sensitivity and accuracy for common liquid assays.
- The system's performance is comparable to commercial spectrometers.
- The GGP-GMR filter's design offers potential for integration into lab-on-a-chip systems for point-of-care applications.
