Microsatellite DNA Analysis for Diversity Study, Individual Identification and Parentage Control in Pig Breeds in...

Swine DNA profiling is of high importance for animal identification and parentage verification. The aim of this study was to test a set of 14 microsatellite (STR) markers recommended by ISAG for parentage verification in Polish Landrace (PL, n = 900), Polish Large White (PLW, n = 482), Pulawska (PUL, n = 127), and Duroc pigs (DU n = 108). The studied breeds showed a medium level of genetic differentiation. The average value of heterozygosity and degree of polymorphism (PIC) were above 0.5 for the studied breeds, except for the DU breed (PIC = 0.477). The population inbreeding coefficient indicates an absence of inbreeding in the studied breeds (an average value of F_IS = 0.007). The cumulative power of discrimination for all breeds reached high values close to 1.0, while the probability of identity (P_ID) was low, with P_ID values ranging between 10^-9 (for DU) and 10^-12 (for PLW). The cumulative exclusion probability for PE₁ and PE₂ showed that the parentage can be confirmed with a probability of from 92.75% to 99.01% and from 99.49% to 99.97%, respectively.