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Updated: Nov 7, 2025

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
12:19

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

Published on: June 27, 2015

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Cytoskeleton Rearrangements Modulate TRPC6 Channel Activity in Podocytes

Alexey Shalygin1, Leonid S Shuyskiy1, Ruslan Bohovyk2

  • 1Institute of Cytology, Russian Academy of Sciences, 194064 Saint-Petersburg, Russia.

International Journal of Molecular Sciences
|April 30, 2021
PubMed

Insights

The kidney

Area of Science:

  • Nephrology and Cell Biology

Background:

  • The actin cytoskeleton in podocytes is crucial for kidney filtration barrier function.
  • Calcium influx via TRPC6 channels influences podocyte actin structure and barrier integrity.

Purpose of the Study:

  • To investigate the feedback mechanism between the podocyte actin cytoskeleton and TRPC6 channel activity.
  • To determine if actin cytoskeleton structure modulates TRPC6 channel function.

Main Methods:

  • Scanning ion-conductance microscopy to assess podocyte migration.
  • Cell-attached patch-clamp electrophysiology to measure TRPC6 channel activity.
  • Co-expression studies in Chinese Hamster Ovary (CHO) cells.

Main Results:

  • Disruption of the actin cytoskeleton with cytochalasin D enhanced TRPC6 channel activity in podocytes and CHO cells.
  • Co-expression of mutant α-actinin-4 (K255E), which solidifies the actin network, decreased TRPC6 channel activity.
  • Demonstrated a direct link between actin cytoskeleton organization and TRPC6 channel regulation.

Conclusions:

  • The actin cytoskeleton directly modulates TRPC6 channel activity, establishing a feedback loop.
  • This interaction is significant for maintaining kidney filtration barrier homeostasis.
Keywords:
FSGSTRPC6actin cytoskeletonfocal segmental glomerulosclerosispodocyteα-actinin-4

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