Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photoelectrochemical Immuno-Sensing via Plasmon-Induced Resonance Energy Transfer Mechanism.

ECS sensors plus·2026
Same author

Honoring the past, preparing for the future: Celebrating 65 years of Latiolais Leadership at Ohio State.

American journal of health-system pharmacy : AJHP : official journal of the American Society of Health-System Pharmacists·2026
Same author

Direct Pattern-to-Curve PDMS-Based Microstructures Fabrication via Thermal Air Expansion for Micro-Optics.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Enhanced Schwann cell differentiation of skin-derived neural crest-like stem cells through the synergistic action of SOX10 and immobilized NRG1 signaling.

Bioengineering & translational medicine·2025
Same author

ASHP National Survey of Pharmacy Practice in Hospital Settings: Clinical Services and Workforce-2024.

American journal of health-system pharmacy : AJHP : official journal of the American Society of Health-System Pharmacists·2025
Same author

Immune-mediated regeneration of cell-free vascular grafts in an ovine model.

NPJ Regenerative medicine·2025

Related Experiment Video

Updated: Nov 7, 2025

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

21.4K

An Integrated Centrifugal Degassed PDMS-Based Microfluidic Device for Serial Dilution.

Anyang Wang1, Samaneh Moghadasi Boroujeni2, Philip J Schneider1

  • 1Sensors and MicroActuators Learning Lab (SMALL), Department of Electrical Engineering, The State University of New York at Buffalo, Buffalo, NY 14260, USA.

Micromachines
|April 30, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microfluidic device for generating serial dilutions using centrifugal and vacuum forces. The device enables precise sample preparation for applications like quantitative polymerase chain reaction (qPCR).

Keywords:
PDMScentrifugal pumpingmicrofluidicqPCRserial dilutionvacuum pumping

More Related Videos

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology
07:03

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Published on: December 1, 2023

1.2K
Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
08:20

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Published on: February 22, 2016

10.6K

Related Experiment Videos

Last Updated: Nov 7, 2025

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

21.4K
Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology
07:03

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Published on: December 1, 2023

1.2K
Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
08:20

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Published on: February 22, 2016

10.6K

Area of Science:

  • Microfluidics
  • Biotechnology
  • Biochemical Engineering

Background:

  • Microfluidic devices offer miniaturization and automation for biological assays.
  • Traditional serial dilution methods can be time-consuming and prone to errors.
  • Integrating multiple forces within microfluidic systems presents opportunities for enhanced functionality.

Purpose of the Study:

  • To develop an integrated serial dilution generator using centrifugal and vacuum-driven forces in a polydimethylsiloxane (PDMS) microfluidic device.
  • To demonstrate the device's capability for precise sample metering, transferring, loading, and mixing.
  • To validate the device's performance for preparing serial dilutions of complementary DNA (cDNA) for quantitative polymerase chain reaction (qPCR).

Main Methods:

  • Fabrication of a microfluidic device using gas-soluble polydimethylsiloxane (PDMS).
  • Integration of centrifugal force for sample loading and mixing.
  • Application of vacuum-driven force for sample manipulation in arbitrary directions, enabling bubble-free operation.
  • Generation of ten-fold serial dilutions from 100 to 10-4 with approximately 8 μL per chamber.

Main Results:

  • The microfluidic device successfully generated serial dilutions with high precision and reproducibility.
  • The hybrid centrifugal and vacuum-driven force system demonstrated robustness and tolerance to surface conditions.
  • Quantitative polymerase chain reaction (qPCR) results from device-prepared dilutions showed good agreement with manual pipetting methods.
  • The serial dilution ratio and chamber volume were easily adjustable through device design modifications.

Conclusions:

  • The proposed integrated serial dilution generator offers a promising platform for automated and precise sample preparation in microfluidic systems.
  • The hybrid force approach overcomes limitations of traditional methods, enhancing efficiency and accuracy for molecular diagnostics.
  • This technology has the potential to streamline workflows in research and clinical settings, particularly for quantitative polymerase chain reaction (qPCR) assays.