Related Experiment Video
Updated: Nov 7, 2025
09:45
An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
Published on: August 18, 2018
11.3K
Top-Down and Bottom-Up Proteomics Methods to Study RNA Virus Biology
Yogy Simanjuntak1, Kira Schamoni-Kast1, Alice Grün1,2
1Leibniz Institute for Experimental Virology (HPI), 20251 Hamburg, Germany.
Viruses
|April 30, 2021
Summary
Mass spectrometry (MS) advances RNA virus research by analyzing host-virus interactions and protein structures. This technology aids in identifying novel antiviral targets for infectious diseases.
Area of Science:
- Virology
- Biochemistry
- Proteomics
Background:
- RNA viruses cause significant human disease burden.
- Viral infections disrupt host cellular processes like proteostasis and macromolecular organization.
- Understanding these disruptions is crucial for developing antiviral strategies.
Purpose of the Study:
- To review mass spectrometry (MS) applications in RNA virus biology.
- To highlight recent MS developments for studying virus-host interactions.
- To explore the potential of MS in identifying new antiviral targets.
Main Methods:
- Review of top-down and bottom-up mass spectrometry techniques.
- Focus on MS applications in characterizing host responses to RNA viruses.
- Integration of structural MS for protein complex analysis.
Main Results:
- Mass spectrometry provides global and structural insights into virus-host perturbations.
- Novel MS tools enable detailed analysis of protein complex stoichiometry and function.
- MS facilitates the identification of critical host factors and viral proteins.
Conclusions:
- Mass spectrometry is a powerful tool in RNA virus research.
- MS-based approaches are vital for understanding viral pathogenesis and host responses.
- Translational applications of MS can accelerate the discovery of novel antiviral therapies.
Related Concept Videos
03:21RNA-seq
10.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.9K
01:33Proteomics
8.7K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.7K
01:29Viruses with RNA Genomes
309
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
309
02:28Leaky Scanning
5.4K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.4K
02:24Ribosome Profiling
3.8K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.8K