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Review of Microfluidic Methods for Cellular Lysis
Emil Grigorov1,2, Boris Kirov2, Marin B Marinov1,3
1Faculty of German Engineering Education and Industrial Management (FDIBA), Technical University of Sofia, 1756 Sofia, Bulgaria.
Micromachines
|April 30, 2021
Summary
This review compares cell lysis methods for DNA/RNA extraction. It details chemical, mechanical, electrical, thermal, and laser techniques, evaluating their pros and cons for effective cell membrane disintegration.
Area of Science:
- Biotechnology
- Molecular Biology
- Biochemistry
Background:
- Cell lysis is crucial for releasing intracellular components like DNA and RNA.
- Effective cell membrane disintegration is essential for molecular analysis.
- Various techniques exist, each with unique technological demands.
Purpose of the Study:
- To comprehensively review and categorize methods for cell lysis.
- To analyze the technological peculiarities and instrumental requirements of each method.
- To compare the advantages and disadvantages of different cell lysis approaches.
Main Methods:
- Categorization of lysis methods into chemical, mechanical, electrical, thermal, laser, and others.
- Review of reported techniques for effective cellular boundaries disintegration.
- Analysis of technological aspects and instrumentation for each method.
Main Results:
- Detailed examination of six distinct categories of cell lysis techniques.
- Identification of specific advantages and disadvantages for each method.
- Comparison of methods based on cost, efficiency, and difficulty.
Conclusions:
- The selection of a cell lysis method is contingent upon specific scientific objectives.
- A comparative table highlights the benefits of each approach for informed decision-making.
- Understanding method-specific characteristics is key to successful molecular analysis.
