Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tissue Homogenization and Cell Lysis01:32

Tissue Homogenization and Cell Lysis

Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring gut microbiota alterations in Parkinson's disease: insights from a 16S amplicon sequencing Eastern European pilot study.

Frontiers in neuroscience·2025
Same author

Linezolid in the Focus of Antimicrobial Resistance of <i>Enterococcus</i> Species: A Global Overview of Genomic Studies.

International journal of molecular sciences·2025
Same author

Linear Interval Approximation of Sensor Characteristics with Inflection Points.

Sensors (Basel, Switzerland)·2023
Same author

Analysis of Polarization Images in the Microphysical Blood Parameters Research for the Hematocrit Diagnostics.

Micromachines·2022
Same author

First detection of a colistin-resistant Klebsiella aerogenes isolate from a critically ill patient with septic shock in Bulgaria.

Acta microbiologica et immunologica Hungarica·2022
Same author

Linear Interval Approximation for Smart Sensors and IoT Devices.

Sensors (Basel, Switzerland)·2022

Related Experiment Video

Updated: Jul 9, 2026

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
09:53

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

Published on: December 31, 2009

12.5K

Review of Microfluidic Methods for Cellular Lysis.

Emil Grigorov1,2, Boris Kirov2, Marin B Marinov1,3

  • 1Faculty of German Engineering Education and Industrial Management (FDIBA), Technical University of Sofia, 1756 Sofia, Bulgaria.

Micromachines
|April 30, 2021
PubMed
Summary

This review compares cell lysis methods for DNA/RNA extraction. It details chemical, mechanical, electrical, thermal, and laser techniques, evaluating their pros and cons for effective cell membrane disintegration.

Keywords:
cell lysiscell membranemicrofluidicsreview

More Related Videos

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
08:02

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

13.1K
Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe
12:19

Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe

Published on: September 15, 2016

7.3K

Related Experiment Videos

Last Updated: Jul 9, 2026

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
09:53

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

Published on: December 31, 2009

12.5K
Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
08:02

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

13.1K
Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe
12:19

Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe

Published on: September 15, 2016

7.3K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biochemistry

Background:

  • Cell lysis is crucial for releasing intracellular components like DNA and RNA.
  • Effective cell membrane disintegration is essential for molecular analysis.
  • Various techniques exist, each with unique technological demands.

Purpose of the Study:

  • To comprehensively review and categorize methods for cell lysis.
  • To analyze the technological peculiarities and instrumental requirements of each method.
  • To compare the advantages and disadvantages of different cell lysis approaches.

Main Methods:

  • Categorization of lysis methods into chemical, mechanical, electrical, thermal, laser, and others.
  • Review of reported techniques for effective cellular boundaries disintegration.
  • Analysis of technological aspects and instrumentation for each method.

Main Results:

  • Detailed examination of six distinct categories of cell lysis techniques.
  • Identification of specific advantages and disadvantages for each method.
  • Comparison of methods based on cost, efficiency, and difficulty.

Conclusions:

  • The selection of a cell lysis method is contingent upon specific scientific objectives.
  • A comparative table highlights the benefits of each approach for informed decision-making.
  • Understanding method-specific characteristics is key to successful molecular analysis.