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Deciphering the Variants Located in the MIR196A2, MIR146A, and MIR423 with Type-2 Diabetes Mellitus in Pakistani

Muhammad Sohail Khan1, Bashir Rahman1, Taqweem Ul Haq1

  • 1Department of Biotechnology, University of Malakand, Chakdara 18800, Pakistan.

Genes
|April 30, 2021
PubMed

Insights

Genetic variations in microRNAs (miRNAs) are linked to Type 2 Diabetes Mellitus (T2DM) risk in Pakistan. Specifically, rs11614913 T/C and rs2910164 G/C variants in miRNA genes are associated with T2DM pathogenesis.

Area of Science:

  • Genetics
  • Molecular Biology
  • Endocrinology

Background:

  • MicroRNAs (miRNAs) are crucial non-coding RNA regulators of gene expression.
  • miRNA gene variations are implicated in various diseases, but their role in Type 2 Diabetes Mellitus (T2DM) in Pakistan is understudied.

Purpose of the Study:

  • To investigate the association of specific miRNA gene variants (rs11614913 T/C, rs2910164 G/C, and rs6505162 C/A) with T2DM in the Pakistani population.
  • To determine the potential of these variants as risk factors for T2DM.

Main Methods:

  • Genotyping of T2DM patients and healthy controls using tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR).
  • Analysis of genotype-phenotype associations using inherited models.

Main Results:

  • The study identified a significant association between the rs11614913 T/C and rs2910164 G/C variants and the risk of developing T2DM.
  • No significant association was found for the rs6505162 C/A variant.

Conclusions:

  • The miRNA gene variants rs11614913 T/C and rs2910164 G/C are linked to T2DM risk in the Pakistani population.
  • These variants may serve as novel risk factors contributing to the pathogenesis of T2DM in this demographic.
Keywords:
MIR146AMIR196A2MIR423MicroRNAT2DMsingle-nucleotide polymorphisms

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