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Microbiological Evaluation and Sperm DNA Fragmentation in Semen Samples of Patients Undergoing Fertility

Chiara Pagliuca1, Federica Cariati2, Francesca Bagnulo3

  • 1Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, via S. Pansini 5, 80131 Napoli, Italy.

Genes
|April 30, 2021
PubMed

Insights

Urogenital infections significantly impact male fertility by reducing semen volume, sperm concentration, and motility. Semen analysis can help diagnose these infections and manage male infertility.

Area of Science:

  • Reproductive Medicine
  • Urology
  • Microbiology

Background:

  • Urogenital infections affect 15% of male infertility cases.
  • Pathogens can disrupt the microenvironment of testes and accessory glands, impairing sperm function.
  • These infections are often asymptomatic, complicating diagnosis and treatment.

Purpose of the Study:

  • To evaluate the impact of urogenital infections on human semen quality.
  • To correlate infection status with specific sperm parameters and DNA integrity.

Main Methods:

  • Standard semen analysis of 53 samples.
  • Microbiological evaluation for pathogen detection.
  • Molecular characterization of sperm DNA fragmentation.

Main Results:

  • A significant correlation was found between infection and reduced semen volume, sperm concentration, and motility.
  • Infected semen samples showed a higher risk of sperm DNA fragmentation.
  • Semen analysis provided indicators for diagnosing and managing urogenital infections in infertile men.

Conclusions:

  • Urogenital infections are a significant factor in male infertility, affecting key sperm parameters.
  • Sperm DNA fragmentation is a notable consequence of these infections.
  • Comprehensive semen analysis is crucial for identifying and managing urogenital infections contributing to male infertility.
Keywords:
male infertilitymicrobiological evaluationsperm DNA fragmentationurogenital infections

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