Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Structural determinants of the scorpion venom peptide Uy234 govern bactericidal activity and membrane-disruptive properties.

Frontiers in microbiology·2026

Same author

A stretch-responsive fibroblast program promotes epidermal stem cell self-renewal during skin expansion.

Nature communications·2026

Same author

Advancing mechanobiology from single molecules to complex cellular systems.

Nature nanotechnology·2026

Same author

International Forum on Visceral Myopathy 2024: Advances in the Knowledge of the Disease.

Neurogastroenterology and motility·2026

Same author

Cell loss disrupts mechanical homeostasis to drive retinal pigment epithelium ageing-like phenotype in vitro.

Nature communications·2026

Same author

The CDKL5 kinase undergoes liquid-liquid phase separation driven by a serine-rich C-terminal region.

Life science alliance·2026

Search research articles

Related Experiment Video

Updated: Nov 7, 2025

Microfluidic Applications for Disposable Diagnostics

Microfluidic Applications for Disposable Diagnostics

Published on: February 3, 2008

An Overview on Microfluidic Systems for Nucleic Acids Extraction from Human Raw Samples.

Daniele Obino¹, Massimo Vassalli², Alberto Franceschi³

¹Institute of Biophysics, National Research Council, 16149 Genova, Italy.

Sensors (Basel, Switzerland)

|April 30, 2021

Summary

Automated nucleic acid (NA) extraction using lab-on-a-chip (LOC) devices offers a faster, user-friendly, and cost-effective alternative to traditional methods. This review details LOC strategies for preparing human biological fluid samples for genetic analysis.

Keywords:

LOC SPE lab-on-chip microfluidics nucleic acid extraction solid-phase extraction

More Related Videos

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

Published on: June 11, 2013

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Published on: August 6, 2016

Related Experiment Videos

Last Updated: Nov 7, 2025

Microfluidic Applications for Disposable Diagnostics

Microfluidic Applications for Disposable Diagnostics

Published on: February 3, 2008

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

Published on: June 11, 2013

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Published on: August 6, 2016

Area of Science:

Biotechnology
Molecular Biology
Genetics

Background:

Nucleic acid (NA) extraction is fundamental for genetic analysis across research, diagnostics, and forensics.
Traditional NA extraction requires specialized facilities, equipment, and extensive labor.
Microfluidic devices, or lab-on-a-chip (LOC) systems, offer potential for efficient and sensitive NA analysis.

Purpose of the Study:

To review existing lab-on-a-chip (LOC) solutions for automated nucleic acid extraction.
To focus on the implementation strategies for preparing human raw biological fluids on-chip.
To highlight the advantages of LOC for portable, rapid, and user-friendly genetic analysis.

Main Methods:

Review of literature on lab-on-a-chip (LOC) devices for NA extraction.
Analysis of strategies for sample preparation from human biological fluids (blood, urine, feces, saliva).
Focus on integration of cell separation, lysis, and NA isolation within microfluidic systems.

Main Results:

Various LOC approaches exist for automated NA extraction from diverse human samples.
Successful integration of sample preparation steps (lysis, isolation) on-chip has been demonstrated.
LOC systems show promise for point-of-care applications due to portability and speed.

Conclusions:

Lab-on-a-chip (LOC) technology is advancing automated nucleic acid extraction from biological fluids.
LOC integration is key to realizing portable, rapid, and cost-effective genetic analysis solutions.
Further development of LOC strategies will enhance accessibility of genetic testing.