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Tissue Homogenization and Cell Lysis

Tissue Homogenization and Cell Lysis

Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...

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Related Experiment Video

Updated: Nov 7, 2025

Using a Pan-Viral Microarray Assay Virochip to Screen Clinical Samples for Viral Pathogens

Using a Pan-Viral Microarray Assay Virochip to Screen Clinical Samples for Viral Pathogens

Published on: April 27, 2011

A Versatile Processing Workflow to Enable Pathogen Detection in Clinical Samples from Organs Using VIDISCA.

Alba Folgueiras-González^1,2, Robin van den Braak¹, Martin Deijs²

¹Department Discovery & Technology, MSD Animal Health, Wim de Körverstraat 35, P.O. Box 31, 5830 AA Boxmeer, The Netherlands.

Diagnostics (Basel, Switzerland)

|April 30, 2021

Summary

We developed a fast, simple method to enrich viral genetic material from tissue samples. This technique improves virus detection sensitivity by reducing host DNA, aiding in the discovery of novel viruses.

Keywords:

VIDISCA-NGS atypical porcine pestivirus enrichment metagenomics virus discovery

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Last Updated: Nov 7, 2025

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Area of Science:

Virology
Molecular Biology
Bioinformatics

Background:

High-throughput sequencing enhances virus discovery in clinical samples.
Host DNA in solid tissues complicates novel virus detection due to low viral abundance.
Physical enrichment methods improve virus detection sensitivity but are often complex and costly.

Purpose of the Study:

To develop and evaluate fast, simple, high-throughput virus enrichment methods for tissue homogenates.
To increase the sensitivity of virus detection using the Virus Identification by Deep Sequencing Analysis (VIDISCA) method.
To reduce host genetic material interference in viral discovery from tissue samples.

Main Methods:

Application of probe sonication in high EDTA concentrations.
Organic extraction using Vertrel™ XF.
Chromatography-based enrichment with Capto™ Core 700 resin.
Combined sonication, extraction, and chromatography enrichment strategies.

Main Results:

Sonication in high EDTA concentrations increased viral reads up to 9.4-fold with minimal host background increase.
A combined method (sonication, Vertrel™ XF extraction, two rounds of Capto™ Core 700 chromatography) achieved up to 10.5-fold increase in viral reads.
The combined method effectively reduced host background sequencing, enhancing viral signal detectability.

Conclusions:

A simple, semi-high-throughput enrichment method was developed for homogenized tissue samples.
This method significantly increases the proportion of viral reads, improving virus detection sensitivity.
The developed technique facilitates the discovery of novel viruses in complex clinical samples by overcoming host DNA interference.