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Related Experiment Video

Updated: Nov 7, 2025

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

Recent advances in rapid antimicrobial susceptibility testing systems.

Michael R Jacobs¹, Jordan D Colson², Daniel D Rhoads³

¹Emeritus Professor of Pathology and Emeritus Medical Director, Clinical Microbiology, Case Western Reserve University and University Hospitals Cleveland Medical Center, Cleveland, OH, USA.

Expert Review of Molecular Diagnostics

|April 30, 2021

Summary

Antimicrobial susceptibility testing (AST) has advanced significantly with new rapid phenotypic and molecular methods. While challenges like cost and throughput remain, these innovations promise more widespread use in clinical settings.

Keywords:

Antimicrobial susceptibility testing MIC bloodstream infections genotype lateral flow assay maldi-tof ms pcr phenotype whole-genome sequencing β-lactamase

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Published on: July 21, 2023

Area of Science:

Clinical Microbiology
Infectious Diseases
Diagnostic Technology

Background:

Traditional antimicrobial susceptibility testing (AST) methods were time-consuming, taking 16-24 hours.
Recent years have seen a significant shift away from these conventional approaches.

Purpose of the Study:

To review recent advances in antimicrobial susceptibility testing (AST).
To discuss the development and impact of rapid phenotypic and molecular methods.
To highlight challenges and future prospects for AST adoption.

Main Methods:

Review of recent developments in phenotypic and molecular AST systems.
Discussion of bacterial identification techniques like MALDI-TOF mass spectrometry.
Examination of PCR arrays for pathogen and resistance gene detection.
Consideration of whole-genome sequencing applications in infectious disease diagnostics.

Main Results:

Numerous rapid phenotypic and molecular AST methods have been developed.
These methods can detect genotypic or phenotypic resistance, including specific markers like beta-lactamases.
MALDI-TOF mass spectrometry is crucial for interpreting rapid AST results.
PCR arrays are available for common bloodstream pathogens and resistance genes.

Conclusions:

The field of AST has undergone substantial transformation in the last decade.
Widespread adoption of rapid AST methods is hindered by high costs and limited throughput.
Continued development, targeted application, and decreasing costs are expected to increase the utilization of these advanced diagnostic tools.