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TCR Vβ Evaluation by Flow Cytometry

Benedetta Peruzzi1, Sara Bencini1, Roberto Caporale2

  • 1Flow Cytometry Diagnostic Center and Immunotherapy (CDCI), AOU Careggi, Florence, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|April 30, 2021
PubMed

T-cell receptor (TCR)-Vβ repertoire analysis is a sensitive method for detection of T-cell clonality. This type of analysis has been used for studying selective T-cell responses in autoimmune disease, alloreactivity in transplantation, and protective immunity against microbial and tumor antigens and in neoplastic T cells. Here, we describe the flow cytometric methods to perform this analysis.

Keywords:
ClonalityClonogramFlow cytometryMonoclonal antibodiesRepertoireT-cell receptor (TCR)-Vβ

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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