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Related Experiment Video

Updated: Nov 7, 2025

Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester

Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester

Published on: June 4, 2019

Quantifying Antigen-Specific T-Cells by Assessing Their Antigen-Induced Proliferation.

Aayushi Shah¹, Hyun-Dong Chang²

¹Deutsches Rheuma-Forschungszentrum Berlin, A Leibniz Institute, Berlin, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|April 30, 2021

Summary

Activated T cells proliferate to expand their numbers. This study details using flow cytometry to measure T cell proliferation at the single-cell level, aiding in the identification of antigen-reactive T cells.

Keywords:

Antigen-specific Cell tracker dyes Proliferation T cells

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Last Updated: Nov 7, 2025

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Published on: June 4, 2019

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Published on: June 19, 2018

Area of Science:

Immunology
Cell Biology

Background:

T cell activation by antigen-MHC complexes is crucial for adaptive immunity.
Activated T cells undergo cell cycle progression and clonal expansion to generate an effective immune response.

Purpose of the Study:

To describe methods for assessing T cell proliferation on a single-cell level.
To demonstrate the utility of flow cytometry in identifying and isolating antigen-reactive T cells.

Main Methods:

Utilizing flow cytometry to analyze T cell proliferation.
Quantifying cell cycle activity and expansion of individual T cells.

Main Results:

Established a flow cytometry-based assay for single-cell T cell proliferation.
Demonstrated the ability to identify antigen-specific T cell populations based on proliferation.

Conclusions:

Flow cytometry provides a powerful tool for dissecting T cell expansion.
This methodology facilitates the isolation of functionally defined, antigen-reactive T cells for further study or therapeutic applications.