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Reproductive Cloning

Reproductive Cloning

Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...

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Related Experiment Video

Updated: Nov 7, 2025

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

Published on: November 21, 2014

Human T-Cell Cloning by Limiting Dilution.

Laura Maggi¹, Manuela Capone², Alessio Mazzoni²

¹Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy. laura.maggi@unifi.it.

Methods in Molecular Biology (Clifton, N.J.)

|April 30, 2021

Summary

This summary is machine-generated.

Generating human T cell clones via limiting dilution culture enables functional studies at the single-cell level. This method supports detailed investigation of T cell properties and functions.

Keywords:

Limiting dilution Single cell T cells TCR

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Area of Science:

Immunology
Cell Biology

Background:

Human T cells exhibit significant heterogeneity in phenotype and function.
Single-cell phenotyping technologies have advanced, but functional analysis remains challenging.
In vitro culture of T cell clones is crucial for single-cell functional studies.

Purpose of the Study:

To describe a reliable method for establishing human T cell clones.
To enable functional investigations of T cells at the single-cell level.
To provide a protocol for maintaining T cell clones in culture.

Main Methods:

Isolation of human T cells.
Establishment of T cell clones using limiting dilution.
Co-culture with feeder cells.
Maintenance of T cell clones in vitro.

Main Results:

Successful generation of human T cell clones.
Demonstration of a reproducible method for T cell cloning.
Establishment of protocols for long-term T cell clone culture.

Conclusions:

Limiting dilution culture is an effective method for obtaining human T cell clones.
This technique facilitates in-depth functional analysis of T cells.
The described method supports ongoing research in T cell immunology.