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Murine T-Cell Transfer Colitis as a Model for Inflammatory Bowel Disease
Patrick Maschmeyer1, Jakob Zimmermann2, Anja Andrea Kühl3
1Deutsches Rheuma-Forschungszentrum (DRFZ) Berlin, an Institute of the Leibniz Association, Berlin, Germany. Patrick.Maschmeyer@drfz.de.
Methods in Molecular Biology (Clifton, N.J.)
|April 30, 2021
Summary
This study details a preclinical model for studying T-cell transfer colitis, crucial for understanding Inflammatory Bowel Disease (IBD) pathogenesis and T helper cell roles in gut inflammation.
Area of Science:
- Gastroenterology
- Immunology
- Preclinical Research
Background:
- Inflammatory bowel disease (IBD) comprises severe chronic gastrointestinal inflammatory conditions.
- Murine models are essential for understanding IBD development and pathogenesis.
- CD4+ T helper cells significantly contribute to IBD pathogenesis.
Purpose of the Study:
- To present a detailed protocol for a preclinical model of T-cell transfer colitis.
- To facilitate the study of T helper cell functions in intestinal inflammation.
- To aid in the understanding of IBD etiology.
Main Methods:
- Development of a T-cell transfer colitis model in mice.
- Standardized protocol for laboratory utilization.
- Focus on CD4+ T helper cell transfer.
Main Results:
- The protocol provides a reproducible method for inducing colitis.
- Enables investigation into the specific roles of T helper cells.
- Offers a valuable tool for IBD research.
Conclusions:
- The T-cell transfer colitis model is an accessible and effective preclinical tool.
- This model aids in dissecting the contribution of T helper cells to intestinal inflammation.
- Facilitates further research into IBD pathogenesis and potential therapies.
