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Long noncoding RNA OIP5-AS1 exhibits oncogenic activity in bladder cancer through miR-217 and MTDH

S-F Zhang1, S Pang, F-P Wang

  • 1Department of Urology Surgery, Caoxian People's Hospital, Heze, China. chichenyan9@163.com.

European Review for Medical and Pharmacological Sciences
|April 30, 2021
PubMed
Abstract

Insights

Long non-coding Opa-interacting protein 5 antisense RNA 1 (OIP5-AS1) promotes bladder cancer progression. Targeting the OIP5-AS1/microRNA-217/metadherin axis inhibits cancer cell proliferation and invasion, suggesting OIP5-AS1 as a potential bladder cancer biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Bladder cancer (BCa) is a significant health concern with complex molecular underpinnings.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development and progression.

Purpose of the Study:

  • To investigate the role of OIP5-AS1 in bladder cancer.
  • To elucidate the molecular mechanism involving OIP5-AS1, microRNA-217 (miR-217), and metadherin (MTDH) in BCa progression.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and Western blot to assess OIP5-AS1, miR-217, and MTDH expression.
  • Cell Counting Kit-8 (CCK-8) and Transwell assays to evaluate BCa cell proliferation and invasion.
  • Luciferase reporter assays and Spearman correlation analysis to determine molecular interactions.

Main Results:

  • OIP5-AS1 expression was significantly upregulated in BCa tissues and cells.
  • Knockdown of OIP5-AS1 suppressed BCa cell proliferation and invasion.
  • OIP5-AS1 directly targets miR-217, which in turn targets MTDH, forming the OIP5-AS1/miR-217/MTDH axis.

Conclusions:

  • OIP5-AS1 promotes bladder cancer cell proliferation and invasion via the miR-217/MTDH pathway.
  • OIP5-AS1 functions as an oncogene in human BCa.
  • OIP5-AS1 holds potential as a diagnostic biomarker and therapeutic target for bladder cancer.

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