Antibacterial potential of selected phytomolecules: An experimental study

Area of Science:

• Microbiology
• Pharmacology
• Natural Products Chemistry

Background:

• Antibiotic resistance is a critical global health threat due to the diminishing effectiveness of existing antibiotics.
• Overuse and underuse of antibiotics have accelerated the development of resistant bacterial strains.
• Novel strategies, including new chemical entities and inhibition of resistance mechanisms, are urgently needed.

Purpose of the Study:

• To investigate the antibacterial activity of selected phytomolecules against various bacterial strains.
• To evaluate the synergistic effects of phytomolecules in combination with standard antibiotics.
• To assess the efficacy and safety of phytomolecules in both in vitro and in vivo models.

Main Methods:

• In vitro assays including microtiter assays, bacterial growth kinetics, and antimicrobial synergy studies.
• In vivo efficacy assessment using a zebrafish infection model.
• Cell viability assays to determine cytoprotective effects.

Main Results:

• Selected phytomolecules demonstrated significant antibacterial activity against both Gram-positive and Gram-negative bacteria.
• Synergistic antibacterial effects were observed when phytomolecules were combined with standard antibiotics.
• Phytomolecules exhibited cytoprotective properties when used in combination with standard antibiotics (SABX).

Conclusions:

• Phytomolecules represent a promising source of novel antibacterial agents.
• Combination therapy with phytomolecules and standard antibiotics could enhance treatment efficacy and combat resistance.
• These findings support the development of phytomolecule-based strategies to address the global challenge of antibiotic resistance.