Leukemic Stem Cell (CD34⁺/CD38^-/TIM3⁺) Frequency in Patients with Acute Myeloid Leukemia: Clinical Implications

This study aimed to address the prognostic relevance of CD34⁺/CD38^-/TIM3⁺ leukemic stem cell (LSC) frequency in patients with acute myeloid leukemia (AML) and its impact on patient outcome. We analyzed the expression of LSC markers (CD34⁺/CD38^-/TIM3⁺) using flow cytometry in bone marrow samples of 53 AML cases before and after induction chemotherapy. The LSC frequency at diagnosis was significantly higher compared with that postinduction (P < .001). Patients were categorized into high LSC expressers (≥ median) and low expressers (< median). Patients with AML with high number of LSCs at diagnosis had significantly lower induction of remission response (P = .0104), shorter disease-free survival, and shorter overall survival (P < .001 for both) compared with those with lower LSC count. Cox regression analysis revealed that LSC frequency at diagnosis is an independent prognostic factor in AML. Assessment of LSCs (CD34⁺/CD38^-/TIM3⁺) at diagnosis is recommended for refining of AML risk stratification.