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Related Concept Videos

Rolling Resistance01:21

Rolling Resistance

438
When a solid cylinder rolls steadily on a rigid surface, the normal force applied by the surface on the cylinder is perpendicular to the tangent at the contact point. However, since no materials are entirely rigid, the surface's reaction to the cylinder involves a range of normal pressures.
For instance, imagine a hard cylinder rolling on a comparatively soft surface. The cylinder's weight compresses the surface beneath it. As the cylinder moves, the material in front of it slows down due to...
438

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Quantitative interpretation of cell rolling velocity distribution.

Adam B Yasunaga1, Yousif Murad2, Vojtěch Kapras3

  • 1Department of Chemistry, The University of British Columbia, Kelowna, British Columbia, Canada; Biochemistry and Molecular Biology, The University of British Columbia, Kelowna, British Columbia, Canada.

Biophysical Journal
|May 1, 2021
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Summary

Investigating leukocyte rolling adhesion, this study reveals a log-normal velocity distribution, offering a more accurate measure of cell motion and adhesion strength than traditional methods.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Immunology

Background:

  • Leukocyte rolling adhesion is crucial for immune response, involving selectin-mediated interactions.
  • Current methods analyze average cell rolling velocity, assuming it reflects adhesion strength.
  • No statistical investigation of instantaneous velocity distribution has been performed.

Purpose of the Study:

  • To develop a method for analyzing instantaneous leukocyte rolling velocity distribution.
  • To demonstrate the statistical distribution of cell velocities under physiological flow.
  • To link velocity distribution to molecular adhesion forces.

Main Methods:

  • Developed a noise-removal technique to accurately measure instantaneous cell velocity.
  • Analyzed velocity distributions at both population and single-cell levels.
  • Correlated velocity distribution parameters with molecular adhesion forces.

Main Results:

  • Revealed that leukocyte rolling velocity follows a log-normal distribution.
  • Demonstrated the log-normal distribution accurately describes cell motion across physiological flow rates.
  • Showed log-normal parameters provide a more precise measure of cell motion than mean/median velocities.

Conclusions:

  • The log-normal distribution accurately characterizes leukocyte rolling velocity.
  • This distribution offers a superior method for assessing cell adhesion dynamics.
  • The P-selectin/PSGL-1 interaction's slip-bond regime explains velocity variations.