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A radioimmunohistochemical method for autoradiographic visualization of cell antigens using 125I-staphylococcal
F M Correa1, S S Guilhaume, J M Saavedra
1Unit on Preclinical Neuropharmacology, National Institute of Mental Health, Bethesda, MD 20892.
Neuroscience Letters
|June 29, 1988
Abstract:
125I-Staphylococcal protein A was used to visualize immunoreactive cell antigen in rat brain and pituitary by autoradiography. Autoradiograms of rat brain sections generated with 125I-protein A were clear and showed low background signals. We were able to visualize neural structures containing tyrosine hydroxylase or methionine-enkephalin-like immunoreactivities in the brain, and vasopressin-like immunoreactivity in the pituitary gland. Our results suggest that 125I-protein A can be used for the radioimmunohistochemical visualization of cell antigens in tissue sections.