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Cholesterol-added antigens can enhance antiglycolipid antibody activity: Application to antibody testing

Yu Hongo1, Kenichi Kaida2, Yukari Komuta1

  • 1Department of Neurology, National Defense Medical College, Tokorozawa, Japan.

Journal of Neuroimmunology
|May 2, 2021
PubMed

We analysed the effect of adding cholesterol to glycolipid antigens on antibody activity with enzyme-linked immunosorbent assay in 123 subjects consisting of 96 patients with Guillain-Barré syndrome, 25 Miller Fisher syndrome, and two Bickerstaff brainstem encephalitis. The use of cholesterol-added GM1 antigens increased anti-GM1 activity in 11 out of 23 anti-GM1-positive patients and resulted in six out of 100 anti-GM1-negative patients becoming anti-GM1-positive. Enhancement of anti-GM1 activity by cholesterol addition was significantly associated with antecedent gastrointestinal infection. The use of cholesterol-added glycolipid antigens can increase the detection rate of anti-glycolipid antibodies and accurately evaluate the anti-glycolipid antibody activity in vivo.

Keywords:
AntibodyCholesterolGangliosideGuillain-Barré syndromeLipid environment

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