Related Experiment Video
Updated: Nov 7, 2025
06:35
Cell Membrane Repair Assay Using a Two-photon Laser Microscope
Published on: January 2, 2018
13.0K
ANO5 in membrane repair - Status: "It's complicated"
Volker Gerke1, Ursula Rescher2
1Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation (ZMBE) and Cells in Motion Interfaculty Center (CiM), University of Muenster, Muenster, Germany.
Cell Calcium
|May 2, 2021
Summary
Annovon (ANO5) gene mutations cause muscle disease. ANO5 deficiency impairs plasma membrane repair and causes calcium (Ca2+) overload, but its precise cellular location remains debated.
Area of Science:
- Molecular biology
- Cell biology
- Genetics
Background:
- Mutations in the ANO5/TMEM16E gene are linked to various myopathies.
- Recent studies highlight ANO5 deficiency's role in cellular dysfunction.
Purpose of the Study:
- To investigate the cellular mechanisms underlying ANO5-associated myopathies.
- To determine the specific cellular compartment where ANO5 functions.
Main Methods:
- Analysis of ANO5-deficient cellular models.
- Assessment of plasma membrane integrity and calcium homeostasis.
- Cellular localization studies.
Main Results:
- ANO5 deficiency leads to impaired plasma membrane repair processes.
- ANO5 deficiency results in intracellular calcium (Ca2+) overload.
- Evidence suggests ANO5 may function at the plasma membrane or endoplasmic reticulum.
Conclusions:
- ANO5 plays a critical role in maintaining plasma membrane integrity and calcium balance.
- Further research is needed to pinpoint the exact location of ANO5 activity.
- Understanding ANO5 function is crucial for developing therapies for ANO5-related myopathies.
Related Concept Videos
01:08Nucleotide Excision Repair
39.4K
Overview
39.4K
01:38Nucleotide Excision Repair
4.3K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
4.3K
00:59Mechanisms of Membrane Domain Formation
3.5K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
Another mechanism for membrane domain formation involves membrane proteins interacting with...
3.5K
02:25Overview of DNA Repair
32.5K
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Chemically...
32.5K
02:25Overview of DNA Repair
8.7K
8.7K
01:22Enlargement of the Plasma Membrane
2.1K
Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...
2.1K