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Updated: Nov 7, 2025

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Published on: January 7, 2019
Investigation of the molecular structure complexity of dissolved organic matter by UPLC-orbitrap MS/MS
Lijie Li1, Xiaocun Zhuo2, Zhi Fang2
1Center of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology, Beijing, 100124, China; State Key Laboratory of Heavy Oil Processing, China University of Petroleum (Beijing), Beijing, 102249, China.
Abstract:
The complex natural organic matter of the Suwannee River fulvic acid (SRFA) standard was analyzed by online reversed-phase chromatography with Orbitrap MS/MS using collision-induced dissociation (CID). The number of isobars per nominal mass could be reduced to a single dominantly abundant species in a chromatographic run, sharing some ions with signals having the identical molecular formula in adjacent chromatographic segments and later serving as a precursor ion for fragmentation. A very large proportion of the same fragment ions existed in adjacent chromatographic fractions. The difference in the fragment ions in adjacent chromatographic fractions could be attributed to a gradual change in the formula composition of precursor ions in a chromatographic run. It could be concluded that dissolved organic matter (DOM) molecules with the same elemental composition in different chromatographic fractions may have very similar molecular structures. In addition, we propose a possible DOM model that might greatly deepen our understanding of the behavior of DOM in aquatic matrices.
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