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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
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Handheld laser-induced fluorescence detection systems with different optical configurations.

Ya-Mei Peng1, Jian-Zhang Pan1, Qun Fang2

  • 1Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China.

Talanta
|May 3, 2021
PubMed
Summary

Researchers developed miniaturized laser-induced fluorescence (LIF) detectors for field analysis. The orthogonal configuration achieved a 10 pM detection limit for sodium fluorescein in a handheld device.

Keywords:
HandheldLaser-induced fluorescenceLimit of detectionOptical arrangementRay-tracing simulation

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Area of Science:

  • Analytical Chemistry
  • Optical Engineering
  • Biomedical Instrumentation

Background:

  • Growing need for portable and miniaturized detection systems in various research fields.
  • Laser-induced fluorescence (LIF) offers sensitive detection capabilities.
  • Existing LIF systems often lack portability and miniaturization.

Purpose of the Study:

  • To develop and optimize miniaturized LIF detectors with different optical configurations.
  • To investigate the performance of orthogonal, confocal, and oblique LIF setups.
  • To create a compact, handheld LIF detector for field applications.

Main Methods:

  • Development of LIF detectors using laser diodes and photodiodes.
  • Computer simulation and experimental analysis of light scattering and fluorescence.
  • Optimization of sample preparation, flow rate, alignment, and filter selection.
  • Fabrication of a fully integrated handheld orthogonal LIF detector.

Main Results:

  • Optimized orthogonal and confocal LIF detectors achieved detection limits of 40 pM and 50 pM for sodium fluorescein, respectively.
  • Oblique LIF detectors showed limits of detection (LOD) of 1 nM (45°) and 7 nM (67.5°).
  • A handheld orthogonal LIF detector (50x20x46 mm³) demonstrated a 10 pM LOD for sodium fluorescein at a cost of $380.

Conclusions:

  • Miniaturized LIF detectors can be effectively developed with various optical configurations.
  • The developed handheld LIF detector is a cost-effective and portable solution for field analysis.
  • These miniaturized LIF systems hold potential for integration into other analytical platforms.