A green deep eutectic solvent modified magnetic titanium dioxide nanoparticles for the solid-phase extraction of...

Area of Science:

• Materials Science
• Analytical Chemistry
• Biochemistry

Background:

• Solid-phase extraction (SPE) is crucial for isolating biomolecules.
• Developing efficient and reusable extraction materials is an ongoing challenge.
• Green chemistry approaches, like using deep eutectic solvents (DES), offer sustainable alternatives.

Purpose of the Study:

• To synthesize and characterize magnetic titanium dioxide nanoparticles modified with a choline chloride-xylitol deep eutectic solvent (Fe3O4@TiO2@[ChCl][Xyl]).
• To evaluate the efficiency of the synthesized material for the solid-phase extraction of chymotrypsin (Chy).
• To optimize experimental parameters for maximizing Chy extraction and assess the material's reusability.

Main Methods:

• Synthesis of Fe3O4@TiO2@[ChCl][Xyl] nanoparticles.
• Characterization using FT-IR, TGA, Zeta potential, XRD, VSM, and TEM.
• Optimization of MSPE parameters (concentration, time, pH, ionic strength, temperature, matrix) for chymotrypsin extraction.

Main Results:

• The Fe3O4@TiO2@[ChCl][Xyl] nanoparticles exhibited enhanced physicochemical properties.
• An optimized extraction capacity of 347.8 mg g⁻¹ for chymotrypsin was achieved.
• An 85.9% elution rate was obtained using a 10% SDS-HAc eluent, with good reusability over six cycles.

Conclusions:

• The Fe3O4@TiO2@[ChCl][Xyl] material demonstrates superior performance for chymotrypsin extraction compared to unmodified nanoparticles.
• The synthesized material shows significant potential for the efficient and sustainable extraction of proteins and other biomolecules.
• This green DES-modified magnetic nanoparticle offers a promising tool for bioseparation applications.