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Related Experiment Video

Updated: Nov 7, 2025

Corneal Donor Tissue Preparation for Endothelial Keratoplasty
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Mini-DSAEK for Macro Corneal Perforations.

Alfonso Vasquez-Perez1, Nizar Din1, Maria Phylactou1

  • 1Cornea & External Diseases Department, Moorfields Eye Hospital, NHS Foundation Trust, London, United Kingdom; and.

Cornea
|May 3, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces mini-Descemet stripping endothelial keratoplasty (EK) to repair corneal macroperforations, preserving vision and avoiding complex surgeries. The technique successfully sealed perforations, offering a viable alternative for patients with poor visual potential.

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Area of Science:

  • Ophthalmology
  • Corneal Surgery
  • Tissue Engineering

Background:

  • Corneal macroperforations pose significant challenges, often requiring tectonic keratoplasty with high risks of poor visual outcomes and graft rejection.
  • Traditional lamellar or penetrating keratoplasty techniques may be unsuitable for eyes with limited visual potential or those at high risk of rejection.

Observation:

  • A novel mini-Descemet stripping endothelial keratoplasty (mini-DESEKT) technique was developed and tested in a wet laboratory setting to address paracentral corneal macroperforations.
  • The technique involves introducing a minidisc graft through the perforation and securing it with a double mattress suture to prevent graft herniation.

Findings:

  • Laboratory tests confirmed mini-DESEKT effectively seals macroperforations without large incisions, preserving ocular integrity.
  • Clinical application in three cases demonstrated successful outcomes, including unaided 20/15 vision in one case and long-term stability in two cases with limbal stem-cell deficiency.

Implications:

  • Mini-DESEKT offers a promising alternative to conventional tectonic keratoplasty for paracentral corneal perforations, potentially improving visual prognosis.
  • This approach facilitates host tissue preservation and may reduce the risk of rejection in high-risk patients, enhancing graft survival.