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Aryl Hydrocarbon Receptor and Autophagy-Related Protein Microtubule-Associated Protein Light Chain 3 Expression in

Jung Eun Kim1, Hye Ran Kim2, Seok Young Kang2

  • 1Department of Dermatology, Soonchunhyang University Cheonan Hospital, Soonchunhyang University College of Medicine, Cheonan, Korea.

Annals of Dermatology
|May 3, 2021
PubMed
Abstract

Insights

The aryl hydrocarbon receptor (AHR) and autophagy are implicated in psoriasis. AHR and autophagy may influence epidermal thickness and inflammation, suggesting therapeutic potential for skin diseases.

Area of Science:

  • Dermatology
  • Molecular Biology
  • Cellular Biology

Background:

  • The aryl hydrocarbon receptor (AHR) and autophagy are crucial for skin homeostasis but also implicated in skin disorders.
  • Their specific roles in the pathogenesis of psoriasis remain largely unknown.

Purpose of the Study:

  • To investigate the immunohistochemical and gene expression of AHR, CYP1A1, and microtubule-associated protein light chain 3 (LC3) in psoriasis lesional skin.
  • To determine correlations between AHR, CYP1A1, and LC3 expression and clinicopathological features of psoriasis.

Main Methods:

  • Analysis of skin biopsies from 24 psoriasis patients and 10 healthy controls.
  • Immunohistochemistry, immunofluorescence, and western blotting for protein expression (AHR, CYP1A1, LC3).
  • Quantitative polymerase chain reaction for mRNA levels (AHR, CYP1A1, LC3, ATG5, BECN1, Nrf2).

Main Results:

  • Elevated AHR and CYP1A1 protein and mRNA expression in psoriasis lesions compared to controls.
  • Reduced LC3 protein and mRNA expression in psoriasis lesions.
  • Positive correlations between AHR/CYP1A1 and epidermal thickness/inflammatory cell density.
  • Negative correlations between LC3 and epidermal thickness/inflammatory cell density.
  • A significant inverse correlation between AHR and LC3 expression in psoriatic skin.

Conclusions:

  • AHR and autophagy signaling pathways are dysregulated in psoriasis.
  • These pathways may contribute to psoriasis pathogenesis by influencing epidermal hyperproliferation and inflammation.
  • Targeting AHR and autophagy presents a potential therapeutic strategy for chronic inflammatory skin diseases like psoriasis.
Keywords:
AutophagyCytochrome P-450 CYP1A1PsoriasisReceptorsaryl hydrocarbon

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