A new method for the artificial raising of infant rats: the palate cannula

Chronic removal of infant rats from their mother prior to the onset of weaning is complicated by the fact that young rats do not easily suckle from an artificial nipple. Thus, a method of artificial raising is advantageous for developmental investigations of nutrition or ingestive behaviors during the suckling period. The intragastric cannula has become a popular method for this purpose. However, for many studies, it would be advantageous if the diet could be administered to the mouth and actually swallowed by the young rat. We developed a new cannulation procedure which accomplishes these goals. Infant rats were removed from their mother on postnatal day 13 and fitted with a cannula that opened into the oral cavity through the hard palate. Liquid diet was administered by an infusion pump through the cannula for the subsequent 5 days. Growth was assessed by daily measures of body and organ weight. The results indicate that from postnatal day 13 on, the palate cannula can allow the continuation of normal growth patterns and eliminates certain complicating factors associated with other forms of artificial raising.