Related Experiment Video

Updated: Nov 7, 2025

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
05:36

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

Published on: February 8, 2020

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RUNX1/miR-582-5p Pathway Regulates the Tumor Progression in Clear Cell Renal Cell Carcinoma by Targeting COL5A1

Jianxin Xue1,2, Shenhao Zhu2, Feng Qi3

  • 1Department of Urology, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing, China.

Frontiers in Oncology
|May 3, 2021
PubMed

Insights

MicroRNA-582-5p suppresses clear cell renal cell carcinoma (ccRCC) growth by targeting COL5A1. RUNX1 negatively regulates miR-582-5p, suggesting the RUNX1/miR-582-5p/COL5A1 pathway as a potential ccRCC therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are implicated in clear cell renal cell carcinoma (ccRCC) progression.
  • The precise molecular mechanisms of miRNAs in ccRCC remain incompletely understood.

Purpose of the Study:

  • To elucidate the role and regulatory mechanism of miR-582-5p in ccRCC.
  • To investigate the potential of miR-582-5p as a biomarker and therapeutic target in ccRCC.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) to assess miR-582-5p expression.
  • In vivo and in vitro experiments to evaluate tumor growth inhibition.
  • Database prediction and chromatin immunoprecipitation to identify regulatory factors.
  • Rescue experiments to validate regulatory relationships.

Main Results:

  • miR-582-5p was found to be downregulated in ccRCC tissues and cell lines.
  • Lower miR-582-5p expression correlated with advanced tumor stage and metastasis.
  • miR-582-5p inhibited ccRCC tumor growth and invasion by suppressing COL5A1.
  • RUNX1 was identified as a negative regulator of miR-582-5p.

Conclusions:

  • miR-582-5p acts as a tumor suppressor in ccRCC by targeting COL5A1.
  • The RUNX1/miR-582-5p/COL5A1 axis represents a potential therapeutic strategy for ccRCC.
  • miR-582-5p may serve as a valuable biomarker for ccRCC prognosis.
Keywords:
Runx1ccRCCinvasionmiR-582-5pproliferation

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