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Carbon-dioxide Fixation01:28

Carbon-dioxide Fixation

246
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
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Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

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During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
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Role of Reduced Coenzymes NADH and FADH₂01:29

Role of Reduced Coenzymes NADH and FADH₂

15.0K
The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
15.0K
Photosystem I01:27

Photosystem I

67.9K
Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
67.9K
The Calvin Benson Cycle01:46

The Calvin Benson Cycle

5.2K
Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
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Chemiosmosis and ATP Synthesis01:22

Chemiosmosis and ATP Synthesis

938
The electron transport chain is a critical component of cellular respiration, occurring in the inner mitochondrial membrane. It facilitates the transfer of high-energy electrons from reduced cofactors NADH and FADH₂ to molecular oxygen, the final electron acceptor. This transfer of electrons through a series of protein complexes is tightly coupled to the translocation of protons across the membrane, generating a proton gradient essential for ATP synthesis.Electron Flow and Proton...
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Updated: Nov 7, 2025

CO2 Photoreduction to CH4 Performance Under Concentrating Solar Light
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CO2 Photoreduction to CH4 Performance Under Concentrating Solar Light

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Light-Driven CO2 Reduction by Co-Cytochrome b 562.

Rafael Alcala-Torano1, Nicholas Halloran1, Noah Gwerder1

  • 1School of Molecular Sciences, Arizona State University, Tempe, AZ, United States.

Frontiers in Molecular Biosciences
|May 3, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed artificial protein catalysts for light-driven carbon dioxide reduction. These catalysts convert CO2 into usable molecules under mild conditions, offering a sustainable solution.

Keywords:
CO2 reductionCarbon fixationcatalysiscobalt porphyrinprotein designproton reduction

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Area of Science:

  • Biocatalysis
  • Green Chemistry
  • Artificial Photosynthesis

Background:

  • Rising atmospheric carbon dioxide concentrations pose significant environmental concerns.
  • Sustainable methods for CO2 reduction to valuable products are in high demand.

Purpose of the Study:

  • To develop artificial protein catalysts for efficient light-driven CO2 reduction.
  • To investigate the role of protein scaffolds and cofactors in enhancing catalytic activity.

Main Methods:

  • Engineered cytochrome b562 protein scaffolds.
  • Incorporation of cobalt protoporphyrin IX as a cofactor.
  • Investigated light-driven CO2 reduction in aqueous solution under mild conditions.

Main Results:

  • The artificial protein catalysts demonstrated efficient CO2 reduction to CO.
  • Incorporation into protein scaffolds enhanced the reactivity of cobalt porphyrin.
  • Mutations around the binding site modulated catalytic activity.

Conclusions:

  • Artificial protein catalysts show promise for sustainable CO2 utilization.
  • Protein engineering can optimize biocatalyst performance for CO2 conversion.
  • Further development through rational design and directed evolution can improve catalytic efficiency.