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Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications
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Covalent functionalization of decellularized tissues accelerates endothelialization.

Eleonora Dal Sasso1, Annj Zamuner2,3, Andrea Filippi3,4,5,6

  • 1Department of Cardiac, Thoracic and Vascular Sciences and Venetian Institute of Molecular Medicine, Padua, Italy.

Bioactive Materials
|May 3, 2021
PubMed
Summary
This summary is machine-generated.

Functionalizing biomaterials with REDV peptide enhances endothelial cell adhesion and proliferation, improving hemocompatibility for tissue regeneration. This method supports blood-contacting applications without altering scaffold properties.

Keywords:
Covalent functionalizationDecellularized aortaDecellularized pericardiumEndothelializationMechanical analysisREDV

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Hemocompatibility of synthetic and biological replacements is limited by the absence of a stable endothelial lining.
  • Biomaterial functionalization can promote selective endothelial cell (EC) adhesion to overcome these limitations.

Purpose of the Study:

  • To selectively functionalize decellularized bovine pericardia and porcine aortas with a REDV tetrapeptide.
  • To evaluate the *in vitro* enhancement of endothelial cell adhesion and proliferation on functionalized scaffolds.

Main Methods:

  • Decellularized tissues were functionalized with REDV peptide at 10-5 M and 10-6 M concentrations.
  • Scaffold-bound peptide was quantified.
  • Human umbilical vein ECs were seeded statically to assess adhesion and proliferation.
  • Histological, geometrical, and mechanical properties were analyzed.

Main Results:

  • Functionalized tissues showed statistically higher viable cells and MTS production compared to controls.
  • REDV peptide immobilization did not affect scaffold histoarchitecture, geometry, or mechanical properties.
  • Selective REDV immobilization effectively accelerated EC adhesion and promoted proliferation.

Conclusions:

  • Selective immobilization of REDV peptide is a viable strategy to enhance endothelialization of decellularized tissues.
  • This approach improves the hemocompatibility of biomaterials for blood-contacting applications.
  • Functionalized scaffolds support EC adhesion and proliferation, crucial for tissue regeneration.