Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Centrifugation01:05

Centrifugation

3.2K
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
3.2K
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

6.6K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
6.6K
Subcellular Fractionation01:32

Subcellular Fractionation

8.1K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
8.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perfluorocarbon-based artificial oxygen carriers in perioperative and surgical care: a scoping review of basic and translational studies.

Frontiers in medicine·2026
Same author

The Genetic Association of rs5746105 Polymorphism of SOD2 with the Susceptibility to Sudden Deafness.

The journal of international advanced otology·2026
Same author

Altered thalamic subregional connectivity with default mode and frontoparietal networks in adolescents with depression.

Journal of affective disorders·2026
Same author

Management of Sepsis-Induced Coagulopathy and Septic Pulmonary Embolism Secondary to Severe Maxillofacial Space Infection.

The Journal of craniofacial surgery·2026
Same author

Serum CAPN1 and CLEC2 as Biomarkers for Hearing Loss Severity and Internal Auditory Artery Narrowing in Sudden Sensorineural Hearing Loss.

International journal of general medicine·2026
Same author

A spatiotemporal dependency-aware lightweight CNN-ViT network for 3D MRF with a balanced acceleration strategy.

Medical image analysis·2026

Related Experiment Video

Updated: Nov 7, 2025

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
11:08

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

Published on: January 7, 2019

21.6K

Microalgae separation by inertia-enhanced pinched flow fractionation.

Saijie Wang1, Zhijian Liu2, Sen Wu2

  • 1School of Science, Dalian Maritime University, Dalian, P. R. China.

Electrophoresis
|May 3, 2021
PubMed
Summary

This study introduces inertia-enhanced pinched flow fractionation (iPFF) for efficient microalgae separation. The method achieves high purity and recovery rates for ballast water detection, improving accuracy.

Keywords:
MicroalgaeMicrofluidic chipSeparationiPFF

More Related Videos

Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry
11:44

Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry

Published on: March 6, 2016

9.5K
Analysis of Fatty Acid Content and Composition in Microalgae
07:44

Analysis of Fatty Acid Content and Composition in Microalgae

Published on: October 1, 2013

60.9K

Related Experiment Videos

Last Updated: Nov 7, 2025

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
11:08

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

Published on: January 7, 2019

21.6K
Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry
11:44

Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry

Published on: March 6, 2016

9.5K
Analysis of Fatty Acid Content and Composition in Microalgae
07:44

Analysis of Fatty Acid Content and Composition in Microalgae

Published on: October 1, 2013

60.9K

Area of Science:

  • Biotechnology
  • Environmental Science
  • Fluid Dynamics

Background:

  • Accurate detection of microalgae in ships' ballast water is crucial for environmental protection.
  • Current methods for microalgae separation can be inefficient and lack precision.
  • Size-based separation is a significant factor in improving detection accuracy and efficiency.

Purpose of the Study:

  • To develop and report a novel method for separating microalgae based on size.
  • To optimize the separation process using inertia-enhanced pinched flow fractionation (iPFF).
  • To lay the foundation for accurate microalgae detection in ballast water.

Main Methods:

  • Utilized inertia-enhanced pinched flow fractionation (iPFF) to separate microalgae.
  • Employed inertial lift forces induced by fluid flow for continuous size-based separation.
  • Investigated the effects of Reynolds number and sheath fluid to sample flow rate ratio on separation performance.

Main Results:

  • Optimal separation was achieved at a Reynolds number of 12.3.
  • Increased sheath fluid to sample flow rate ratio improved separation, with the best results at a ratio of 10.
  • Achieved high recovery (99%) and purity (99%) for Chlorella sp., and good recovery (90%) and purity (86%) for Tetraselmis sp.

Conclusions:

  • iPFF offers a simple and effective method for separating microalgae by size.
  • The optimized iPFF method significantly enhances the accuracy and efficiency of microalgae detection.
  • This technique provides a foundational advancement for monitoring and managing ballast water quality.