Hollow POM@MOF-derived Porous NiMo₆ @Co₃ O₄ for Biothiol Colorimetric Detection

Developing highly active and sensitive peroxidase mimics for _L -cysteine (_L -Cys) colorimetric detection is very important for biotechnology and medical diagnosis. Herein, polyoxometalate-doped porous Co₃ O₄ composite (NiMo₆ @Co₃ O₄ ) was designed and prepared for the first time. Compared with pure and commercial Co₃ O₄ , NiMo₆ @Co₃ O₄ (n) composites exhibit the enhanced peroxidase-mimicking activities and stabilities due to the strong synergistic effect between porous Co₃ O₄ and multi-electron NiMo₆ clusters. Moreover, the peroxidase-mimicking activities of NiMo₆ @Co₃ O₄ (n) composites are heavily dependent on the doping mass of NiMo₆ , and the optimized NiMo₆ @Co₃ O₄ (2) exhibits the superlative peroxidase-mimicking activity. More importantly, a sensitive _L -Cys colorimetric detection is developed with the sensitivity of 0.023 μM^-1 and the detection limit at least 0.018 μM in the linear range of 1-20 μM, which is by far the best enzyme-mimetic performances, to the best our knowledge.