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α-Linolenic acid induced TM4 Sertoli cells proliferation and enhanced total antioxidant capacity
Wanhong Li1, Yongyu Yao1, Xiuiu Weng1
1Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; Engineering Research Center of Grassland Industry, Ministry of Education; State Key Laboratory of Grassland Agro-ecosystems; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
Abstract:
The present study was undertaken to elucidate the direct ALA effects on mice TM4 Sertoli cells proliferation in vitro. Our results showed that TM4 cells viability was significantly stimulated by ALA (p < 0.05). The 50 μM ALA increased the concentration of total antioxidant capacity, induced the mitochondrial membrane hyperpolarized, and markedly decreased the number of apoptosis cells (p < 0.05). ALA also up-regulated G2/Mitotic-specific cyclin-B1 gene and apoptosis suppressive gene Bcl2 expression (p < 0.05). In conclusion, those results indicated that ALA could increase TM4 Sertoli cells antioxidant capacity, induced the mitochondrial membrane hyperpolarized, inhibited cells apoptosis and stimulated TM4 Sertoli cells proliferation in vitro.
