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Related Experiment Video

Updated: Nov 7, 2025

Author Spotlight: Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT) for NonInvasive Whole-Brain Imaging of Ischemic Stroke
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Integrative Multi-omics Analysis to Characterize Human Brain Ischemia.

Laura Ramiro1, Teresa García-Berrocoso2,3, Ferran Briansó4

  • 1Neurovascular Research Laboratory, Vall D'Hebron Institute of Research (VHIR), Hospital Vall D'Hebron, Universitat Autònoma de Barcelona, Pg. Vall d'Hebron 119-129, 08035, Barcelona, Spain.

Molecular Neurobiology
|May 3, 2021
PubMed
Summary

This study integrated proteomics and transcriptomics to uncover molecular changes in the human brain after stroke. Key proteins and genes were identified, with some showing potential as blood biomarkers for ischemic stroke.

Keywords:
Integrative analysisIschemic strokeProteomicsTranscriptomics

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genomics
  • Proteomics

Background:

  • Stroke remains a leading cause of death and disability worldwide.
  • Understanding stroke pathophysiology is crucial for developing effective treatments.
  • High-throughput omics approaches offer potential for deeper molecular insights into stroke.

Purpose of the Study:

  • To identify protein and gene expression changes in the human brain following ischemia using an integrative omics approach.
  • To explore the translational potential of identified molecular changes in blood samples from ischemic stroke patients.

Main Methods:

  • Proteomics and transcriptomics were performed on human brain samples from stroke patients, comparing infarct core to contralateral regions.
  • Integrative bioinformatics analyses combined proteomics and transcriptomics data to identify dysregulated pathways and key molecules.
  • Validated key gene and protein candidates using orthogonal techniques in independent brain samples.
  • Assessed circulating levels of validated proteins in blood samples from ischemic stroke patients.

Main Results:

  • Identified 128 proteins and 2716 genes significantly dysregulated in the ischemic human brain.
  • Integrative analysis highlighted key molecular pathways and influential molecules.
  • Validated 11 specific molecules (NCDN, RAB3C, ST4A1, DNM1L, A1AG1, A1AT, JAM3, VTDB, ANXA1, ANXA2, IL8) in independent samples.
  • Detected fluctuations of A1AG1 and A1AT in the blood of ischemic stroke patients within the first week post-onset.

Conclusions:

  • The study expands the understanding of ischemic stroke pathology at the molecular level.
  • Identified key molecules warrant further investigation as potential biomarkers or therapeutic targets for stroke.
  • Demonstrated the translational potential of brain-based omics findings to blood biomarkers.