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Updated: Nov 7, 2025

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

Published on: November 4, 2016

Genomics of Staphylococcus aureus ocular isolates.

William L Johnson¹, Michael B Sohn², Samantha Taffner³

¹Department of Ophthalmology, University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America.

|May 3, 2021

Summary

Staphylococcus aureus ocular infections are linked to specific strains and virulence factors. Whole genome sequencing revealed enterotoxins on mobile islands may enhance S. aureus virulence in the eye.

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Last Updated: Nov 7, 2025

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Published on: May 1, 2021

Area of Science:

Microbiology
Genomics
Ophthalmology

Background:

Staphylococcus aureus is a leading cause of ocular infections and vision loss.
Limited understanding exists regarding S. aureus strains and virulence factors specific to ocular infections.
Whole genome sequencing (WGS) offers a comprehensive approach to study strain types and virulence factors in clinical isolates.

Purpose of the Study:

To characterize S. aureus ocular clinical strains using WGS.
To identify common strain types (MLST, spa, agr) and virulence factors in ocular isolates.
To compare ocular isolates with non-ocular S. aureus strains to find key differences.

Main Methods:

Sequencing of 163 S. aureus ocular clinical strains.
Assessment of common strain types (MLST, spa, agr) and 235 virulence factors.
Comparison with 116 non-ocular S. aureus strains from NCBI.

Main Results:

Common sequence types (ST5, ST8, ST30) in ocular isolates mirror non-ocular pathogenic strains.
Ocular isolates showed significant enrichment for enterotoxins.
Enterotoxins were found on mobile pathogenicity islands, suggesting horizontal gene transfer.

Conclusions:

Ocular S. aureus strains share common types with non-ocular strains.
Enterotoxins may play a crucial role in S. aureus ocular pathogenicity.
Horizontal gene transfer potentially increases S. aureus virulence in ocular tissues.