Glassware Calibration
Testing a Claim about Standard Deviation
You might also read
Articles linked to this work by shared authors, journal, and citation graph.
Updated: Nov 7, 2025

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
Published on: April 11, 2016
Nicholas J Bevins1, Daniel R Luevano2, Robin Nuspl3
1Department of Pathology, University of California at San Diego, San Diego, CA, USA.
This study introduces a new method to identify and reduce unnecessary laboratory testing. Researchers analyzed test volume ratios to detect sites with abnormal testing patterns. They used these ratios to target interventions at outlier sites. The results showed significant reductions in low-value testing at some sites. The approach provides a scalable solution for improving diagnostic stewardship. The study demonstrates that data-driven strategies can effectively reduce unnecessary testing. The method uses benchmarking to guide targeted interventions. This could help healthcare systems reduce costs and improve patient care.
Area of Science:
Background:
Current healthcare systems often face challenges in optimizing diagnostic test utilization. While prior research has established that unnecessary laboratory testing increases costs and risks, gaps remain in identifying and addressing low-value testing at the population level. Existing studies have focused on individual test frequencies but lack methods to account for variations in patient volume across different sites. This gap motivated the development of a novel approach using test volume ratios to detect outliers. Prior work has not explored how benchmarking these ratios could guide targeted interventions. The Choosing Wisely guidelines have highlighted specific analytes for scrutiny, but their implementation has lacked data-driven precision. This study introduces a new framework to identify and reduce low-value testing through standardized ratio analysis. The approach allows for site-specific benchmarking, which has not been widely adopted in diagnostic stewardship. This method provides a scalable solution to a persistent problem in clinical laboratories.
Purpose Of The Study:
The aim of this study was to develop and test a method for identifying and reducing low-value laboratory testing. The researchers sought to address the problem of unnecessary diagnostic testing by analyzing test volume ratios. They focused on analytes included in the Choosing Wisely guidelines, which are known to be frequently overused. The motivation was to create a scalable and data-driven approach to diagnostic stewardship. The study aimed to identify outlier sites with abnormal test volume ratios. It also aimed to evaluate the effectiveness of targeted interventions at those sites. The goal was to demonstrate that benchmarking test volume ratios could lead to measurable reductions in low-value testing. The study sought to provide a replicable model for other institutions to follow.
Main Methods:
The researchers analyzed test volume data for 17 analytes paired with sodium and 8 pairs of analytes. Data were collected from 108 national sites after appropriate cleaning procedures. Test volume ratios were calculated to account for variations in patient volume across sites. Outliers were defined using these ratios to identify sites with abnormal testing patterns. The Choosing Wisely guidelines provided a framework for selecting analytes for analysis. Interventions were then targeted at outlier sites to reduce low-value testing. The efficacy of these interventions was tracked through changes in test volume ratios. The study used a combination of benchmarking and site-specific interventions to evaluate diagnostic stewardship strategies.
Main Results:
The analysis identified a site with an abnormally high Clostridium difficile/sodium ratio. An intervention at this site led to a 71% decrease in C difficile tests. Two different interventions were applied to sites with elevated CKMB/troponin ratios. One site saw an 11% reduction in CKMB testing while the other saw a 98% reduction. These results demonstrated the effectiveness of targeted interventions. The use of test volume ratios enabled the identification of outlier sites. The approach allowed for precise benchmarking of diagnostic utilization. The interventions were tailored to the specific testing patterns observed at each site. The study showed that reducing low-value testing is achievable through data-driven strategies.
Conclusions:
The authors propose that test volume ratio analysis is a useful tool for identifying low-value laboratory utilization. They suggest that benchmarking these ratios enables targeted diagnostic stewardship. The study demonstrates that interventions can effectively reduce unnecessary testing at outlier sites. The results support the use of test volume ratios as a scalable solution. The authors suggest that this approach could be applied to other analytes and sites. They propose that the method provides a data-driven alternative to traditional monitoring. The study shows that different interventions can lead to varying degrees of success. The authors suggest that this approach could help reduce healthcare costs and improve patient outcomes.
Test volume ratio benchmarking compares the number of specific tests to a reference analyte like sodium. This method helps detect sites with abnormal testing patterns, indicating potential low-value utilization.
The Choosing Wisely guidelines highlight frequently overused tests. The study used these guidelines to select analytes for analysis, focusing on those known to be overutilized in clinical practice.
Sodium was used to correct for variations in patient volume across sites. This allowed for a fair comparison of test utilization rates between different locations.
The study applied two different interventions at two sites. One led to an 11% reduction in CKMB tests, while the other achieved a 98% reduction, showing the effectiveness of tailored approaches.
The most significant reduction was a 98% decrease in CKMB testing at one site following an intervention, demonstrating the potential of targeted strategies.
The study introduces a data-driven method for identifying and reducing low-value testing. It shows that benchmarking test volume ratios can guide effective interventions to improve diagnostic utilization.